Journal: Sensors (Basel, Switzerland)

Article Title: Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water

doi: 10.3390/s131115085

Figure Lengend Snippet: Commercial antibodies against cyanobacterial and some marine toxins ^† .

Article Snippet: Microcystin LR , Mybiosource , Mouse, B762M, B764M , –.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water

doi: 10.3390/s131115085

Figure Lengend Snippet: One hundred and fifty repetitive sensor cycles in absence of microcystin. Reprinted with permission from Copyright 2009 Elsevier.

Article Snippet: Microcystin LR , Mybiosource , Mouse, B762M, B764M , –.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water

doi: 10.3390/s131115085

Figure Lengend Snippet: Long-term measurements of microcystin-LR in Lake Tai, China. Reprinted with permission from . Copyright 2013 American Chemical Society.

Article Snippet: Microcystin LR , Mybiosource , Mouse, B762M, B764M , –.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water

doi: 10.3390/s131115085

Figure Lengend Snippet: Setup of a fiber-optical immunosensor for the parallel detection of microcystin-LR and TNT. Reprinted with permission from . Copyright (2010) Elsevier.

Article Snippet: Microcystin LR , Mybiosource , Mouse, B762M, B764M , –.

Techniques:

Journal: PLoS ONE

Article Title: Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight

doi: 10.1371/journal.pone.0192643

Figure Lengend Snippet: Primer sequences and product sizes.

Article Snippet: The following specific primary monoclonal antibodies were used at the dilutions recommended by the manufacturers to determine the levels of each protein: mouse antibodies against beta-actin (diluted 1:300), gamma-actin (diluted 1:100), alpha-actinin-1 (diluted 1:100), alpha-actinin-4 (diluted1:100), desmin (diluted 1:300) (Santa Cruz Biotechnology, Inc., USA), and rabbit antibodies against beta-tubulin (diluted 1:1000) (Pierce Thermo Scientific, Inc., USA).

Techniques: Sequencing, Methylation, Histone Deacetylase Assay

Journal: PLoS ONE

Article Title: Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight

doi: 10.1371/journal.pone.0192643

Figure Lengend Snippet: (A) Alpha-actinin-1. (B) Alpha-actinin-4. (C) Beta-actin. (D) Gamma-actin. (E) Beta-tubulin. (F) Desmin. “B”–basal control group, “V”–vivarium control group, “G”–ground control group (level marked by dotted line), “F”–flight group. *–p < 0.05 in comparison with group “G”. The values used to build graphs represented in the . There were no changes in the cytoskeletal proteins contents in the membrane fraction of the cardiac tissue. In the cytoplasmic fraction, alpha-actinin-1 and alpha-actinin-4 protein content decreased during space flight.

Article Snippet: The following specific primary monoclonal antibodies were used at the dilutions recommended by the manufacturers to determine the levels of each protein: mouse antibodies against beta-actin (diluted 1:300), gamma-actin (diluted 1:100), alpha-actinin-1 (diluted 1:100), alpha-actinin-4 (diluted1:100), desmin (diluted 1:300) (Santa Cruz Biotechnology, Inc., USA), and rabbit antibodies against beta-tubulin (diluted 1:1000) (Pierce Thermo Scientific, Inc., USA).

Techniques: Control, Comparison, Membrane

Journal: PLoS ONE

Article Title: Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight

doi: 10.1371/journal.pone.0192643

Figure Lengend Snippet: (A) Alpha-actinin-1. (B) Alpha-actinin-4. (C) Beta-actin. (D) Gamma-actin. (E) Beta-tubulin. (F) Desmin. “B”–basal control group, “V”–vivarium control group, “G”–ground control group (level marked by dotted line), “F”–flight group. The values used to build graphs represented in the . There were no changes of cytoskeletal proteins contents in the membrane and cytoplasmic fractions of the lung tissue.

Article Snippet: The following specific primary monoclonal antibodies were used at the dilutions recommended by the manufacturers to determine the levels of each protein: mouse antibodies against beta-actin (diluted 1:300), gamma-actin (diluted 1:100), alpha-actinin-1 (diluted 1:100), alpha-actinin-4 (diluted1:100), desmin (diluted 1:300) (Santa Cruz Biotechnology, Inc., USA), and rabbit antibodies against beta-tubulin (diluted 1:1000) (Pierce Thermo Scientific, Inc., USA).

Techniques: Control, Membrane

Journal: PLoS ONE

Article Title: Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight

doi: 10.1371/journal.pone.0192643

Figure Lengend Snippet: (A) The actin isoforms, the microfilaments component; desmin, the intermediate filaments component; subunit 2B of beta-tubulin, the microtubules component. (B) The actin-binding proteins. (C) The tubulin-binding proteins. (D) The metabolic proteins, cytochrome c and glyceraldehyde 3-phosphate dehydrogenase. “B”–basal control group, “V”–vivarium control group, “G”–ground control group (level marked by dotted line), “F”–flight group. *–p < 0.05 in comparison with group “G”. The values used to build graphs represented in the .

Article Snippet: The following specific primary monoclonal antibodies were used at the dilutions recommended by the manufacturers to determine the levels of each protein: mouse antibodies against beta-actin (diluted 1:300), gamma-actin (diluted 1:100), alpha-actinin-1 (diluted 1:100), alpha-actinin-4 (diluted1:100), desmin (diluted 1:300) (Santa Cruz Biotechnology, Inc., USA), and rabbit antibodies against beta-tubulin (diluted 1:1000) (Pierce Thermo Scientific, Inc., USA).

Techniques: Binding Assay, Control, Comparison

Journal: PLoS ONE

Article Title: Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight

doi: 10.1371/journal.pone.0192643

Figure Lengend Snippet: (A) The actin isoforms, the microfilaments component; desmin, the intermediate filaments component; subunit 2B of beta-tubulin, the microtubules component. (B) The actin-binding proteins. (C) The tubulin-binding proteins. (D) The metabolic proteins, cytochrome c and glyceraldehyde 3-phosphate dehydrogenase. “B”–basal control group, “V”–vivarium control group, “G”–ground control group (level marked by dotted line), “F”–flight group. *–p < 0.05 in comparison with group “G”. The values used to build graphs represented in the .

Article Snippet: The following specific primary monoclonal antibodies were used at the dilutions recommended by the manufacturers to determine the levels of each protein: mouse antibodies against beta-actin (diluted 1:300), gamma-actin (diluted 1:100), alpha-actinin-1 (diluted 1:100), alpha-actinin-4 (diluted1:100), desmin (diluted 1:300) (Santa Cruz Biotechnology, Inc., USA), and rabbit antibodies against beta-tubulin (diluted 1:1000) (Pierce Thermo Scientific, Inc., USA).

Techniques: Binding Assay, Control, Comparison

Journal: Scientific Reports

Article Title: Pioglitazone Modulates the Vascular Contractility in Hypertension by Interference with ET-1 Pathway

doi: 10.1038/s41598-019-52839-6

Figure Lengend Snippet: Contribution of NFκB to the ET-1-induced oxidative stress and COX-2 expression in VSMC from hypertensive rats. Effect of lactacystin (Lac, 10 µM) on NOX-1 mRNA levels ( a ), NADPH oxidase activity ( b ), COX-2 protein expression ( c ) and mRNA levels ( d ) induced by ET-1 (0.1 µM, 1 h). ( e ) Effect of ET-1 (0.1 µM, 45 min) on nuclear p65 NFκB protein expression in VSMC from SHR; a representative blot of the cytosolic (Cy) and nuclear (Nu) expression is shown below; TATA-binding protein (TBP) and GAPDH (after reblotting) cytosolic and nuclear expressions are also shown to guarantee the successful cellular fractioning. ( f ) Representative photomicrographs of p65 NFκB immunofluorescence (red) in VSMC from SHR in control and after incubation with ET-1 (0.1 µM, 45 min, n = 7). Negative controls without primary or secondary antibody are also shown. Bar scale represents 50 µm. Statistical analysis by Student’s t-test; * P < 0.05 vs control and # P < 0.05 vs ET-1. n denotes number of experiments. Full-length blots are presented in Supplementary Fig. .

Article Snippet: Proteins were separated by 10% SDS-PAGE and transferred to polyvinyl difluoride membranes that were incubated with rabbit polyclonal antibodies for COX-2 (1:250; Cayman Chemical, Ann Arbor, MI, USA, catalogue number 160106, lot 0439169–1), NOX-1 (1:1,000; Abcam, Cambridge, UK, catalogue number ab131088, lot GR3244226-4), p65 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA, catalogue number sc-372, lot BO513) or p-JNK1/2 (1:1,000, Cell Signaling Technology, Danvers, MA, USA, catalogue number #9251, lot 24).

Techniques: Expressing, Activity Assay, Binding Assay, Immunofluorescence, Control, Incubation

Journal: Scientific Reports

Article Title: Pioglitazone Modulates the Vascular Contractility in Hypertension by Interference with ET-1 Pathway

doi: 10.1038/s41598-019-52839-6

Figure Lengend Snippet: Effect of pioglitazone on the role of NFκB and AP-1 to the ET-1-induced COX-2 expression in VSMC from hypertensive rats. ( a ) Effect of pioglitazone (Pio, 10 µM, 18 h) on ET-1 (0.1 µM, 45 min)-induced nuclear p65 NFκB protein expression in vascular smooth muscle cells (VSMC) from SHR; the representative blot of the cytosolic (Cy) and nuclear (Nu) expression is shown in Fig. . ( b ) Representative photomicrographs of p65 NFκB immunofluorescence (red) in VSMC from SHR after incubation with ET-1 (0.1 µM, 45 min) in the absence and in the presence of pioglitazone. Bar scale represents 50 µm. Effect of pioglitazone on ( c ) p-JNK1/2 protein expression (a representative blot is shown in the upper pane; lower panel shows reblotting of upper panel with JNK2 antibody) and ( d ) c-jun mRNA levels induced by ET-1 (0.1 µM, 15 min for p-JNK2 and 1 h for c-jun). Statistical analysis by Student's t-test. n denotes number of experiments. Full-length blots are presented in Supplementary Fig. .

Article Snippet: Proteins were separated by 10% SDS-PAGE and transferred to polyvinyl difluoride membranes that were incubated with rabbit polyclonal antibodies for COX-2 (1:250; Cayman Chemical, Ann Arbor, MI, USA, catalogue number 160106, lot 0439169–1), NOX-1 (1:1,000; Abcam, Cambridge, UK, catalogue number ab131088, lot GR3244226-4), p65 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA, catalogue number sc-372, lot BO513) or p-JNK1/2 (1:1,000, Cell Signaling Technology, Danvers, MA, USA, catalogue number #9251, lot 24).

Techniques: Expressing, Immunofluorescence, Incubation